The tube is the first thing that I would check. One of the good things about the Tube Pre is that it uses a VERY common tube - the 12ax7. If you happen to have access to a guitar amp with a 12ax7, you can just pull the tube out of the amp and try it with the Tube Pre before you spend any money on a replacement.
If that doesn't work, you can open a support ticket at support.presonus.com and our guys will be glad to help further troubleshoot or set up a repair ticket for you.
Hope this helps.
VP of Sales, PreSonus Audio Electronics
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