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I checked the Audiobox USB 96 manual and didn't see any support for Loopback listed which is typically required for streaming. PreSonus has other audio interfaces that do. You can still stream by adding a second audio interface or virtual mixer software like Voicemeeter:

https://vb-audio.com/Voicemeeter/banana.htm

hello thank you for your answer, i have another question, does Studio One 5 have a mixer like that ?

Studio One has a mixer but you'll still need what I said in my previous response for streaming.

Please describe what you specifically want to stream...

hello i'am a musician and i want to stream music impro with my bass and backings tracks on Studio One 5

francisgosselin1 wrotehello i'am a musician and i want to stream music impro with my bass and backings tracks on Studio One 5

Nice!
Here's a good article that describe what's needed. Be sure to check out the Loopback section as it provides details on what it provides and some of the audio interfaces that supports it. The ioStation 24c, Revelator io24 and io44 are new products that also support Loopback for streaming applications.
https://www.presonus.com/learn/technical-articles/Setting-Up-a-Podcasting-Studio

Hello again i realy need help iam not good to this thing i have change my pc for a macbook air modèle : MacBook Air
Identifiant du modèle : MacBookAir10,1
Puce : Apple M1
Nombre total de cœurs : 8 (4 de performance et 4 d’efficacité)
Mémoire : 8 Go

now i want to know if voicemeeter is compatible ? i have install BlackHole 2ch but when its not working. i want the Ampire effects with my bass but when i stream with i cant hear Ampire can you help me please ?

No, Voicemeeter is for Windows only.
Did you try it with your PC before changing to the Apple M1?

ok i have change my gear for this

Processeur Intel(R) Core(TM) i5-1035G1 CPU @ 1.00GHz 1.19 GHz turbo(3,12 GHz)
Mémoire vive installée 20,0 Go (19,8 Go utilisable)
Type du système Système d’exploitation 64 bits, processeur x64

i reinstall SO5 and OBS Studio, all is good, but i want to know when i stream why i dont have the effect of EMPIRE in live stream? must i have a track record ? i dont whant record anyting just do in live process with out recording, can i ? all i can hear it's my flat bass clean nothing else,