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I have to admit, this one truly has me stumped. I was working with a composition this morning and everything was fine, but this evening, when I came back to it, it was just off and weirdly just out of tune. I put the tuner plugin on one of my virtual instruments and when I hit my A and expected to see 440, I was getting more like 435. Out of the 6 channels I had, 5 out of 6 were off in a similar fashion. I restarted both my computer and Studio One and loaded up a new song, brought in a good piano VST and tuner, hit A and got my expected 440 and kept on banging on the key and watched it suddenly detune to about 435 or so and the fine tuner on the VST did not move at all. I replicated this experiment several times with the same result. What the heck could be making my VST detune?

Mystery solved. After more testing, I narrowed down the problem to my MIDI controller, as in the problem only happens when the controller is connected to the VST. I loaded up MidiView and without me touching anything, I saw where the pitch wheel was sending random values. I "exercised" the wheel and it appeared to stop the spurious activity. Who would have thought!

What controller are you using? Is it possible it is sending out some sort of pitch bend command. Is there any form of factory reset for your controller. If so try resetting it. OK I see you have sorted it. It also means the pitch bend on your controller may be dirty etc or faulty.

Some controllers have calibration procedures as well.

I have been facing this issue with Virtual instruments detuning especially and it is really slowing down the production process. How can we resolve this?

Welcome to the Forum!

Seems like you could make an impact instrument with pitches detuned, never tried it but seems like it would work.

That's the key, get instruments that are already detuned to the scale you want:

Search for vst plugin indian scales
Lot of free ones.

Interesting reading:
https://forum.scalerplugin.com/t/indian-ragas/5841/3

How are you going about this detuning now?

Good to put your specs in your signature, click below in my signature.

Check if all involved sample rates (Studio One song / audio interface) are correct and matching.

Sorry, got the wrong answer. I don't look at old threads, just new posts.

you would have to describe this detuning, when does it happen and with what instruments does it happen etc.

Haven't seen a problem with detuning myself.

nkwainjohnpaulsam wroteI have been facing this issue with Virtual instruments detuning especially and it is really slowing down the production process. How can we resolve this?

Virtual instrument don't go out of tune by themselves. Never.

So either you have a MIDI controller that sends pitch bend data or the sample rate is wrong which makes your whole song play go out of tune.

nkwainjohnpaulsam wroteI have been facing this issue with Virtual instruments detuning especially and it is really slowing down the production process. How can we resolve this?

No information here! What controller are you using. Lukas is right. Virtual instruments do not go out of tune. Unless they are getting some form of rouge pitch bend data.

Check the manual for your controller. If there is any form of factory reset then do it. I have among others an MAudio Axiom 61. It was behaving badly for a while then I factory reset it and all was well.

Open the midi monitor as well in Studio One and observe any incoming messages that may not be meant to be. eg pitch band data.

I don't use a controller. I simply work with the Piano Roll. That is why I'm wondering myself. or is it due to sample rate?

nkwainjohnpaulsam wroteI don't use a controller. I simply work with the Piano Roll. That is why I'm wondering myself. or is it due to sample rate?

What instrument? Your issue could be a whole other source of factors. Best to describe it in detail. I see the OP resolved his is, so ok to proceed.

First, list your specs for the most reliable answers. Not that it's crucial here, but the instruments and samples used can often help solve the issue. Thx.

Some thoughts....
Some samplers allow the sample rate to govern the pitch by removing the tuning of the initial or pitch specified sample(s). So that comes back to the pitch change doesn't change on its own (point given, earlier). Typically, it wouldn't be a sample rate issue unless the instrument (of which I don't know which) would allow for it.

You can see where your issue may be then entirely different.

If the samples are from Presence XT, There are some patches that are in another key. So we need to know the issue more clearly.

Side note; (choose a controller)
Check out a Korg Nano controller if table space is an issue. better yet, an Atom SQ to speed up workflow. Drawing notes in a piano roll is all too time consuming. Of course, I'm guilty of drawing many notes in myself.

Pitch issues in general.
You may also want to check any LFO or pitch modulation settings. LFO (Low Frequency Oscillator) are very subtle and can produce such pich variences. LFO's typically have a waver in pitch. Pitch filters are steady depending on the wave envelope drawn. Did you redraw any wave envelopes? Not amplitude envelopes; but pitch. Even a slight change can cause pitch to vary from some sample instrumemts more than others. Check pitch controller settings as well. You wouldn't need a controller to have unexpectedly altered your pitch.

Check the Inspector track under pitch and tune settings. I can't emphasize enough how moving by those fields with a mouse can alter your settings.

Lastly, I'd wring out (remove) all such parts in the song and then listen. Only dont save. Just try to isolate the problem so that in case you saved the patch, it won't come back.