Strange question, but bear with me....since you don't have your system specs in your signature....
First, are you PC or MAC? 2nd, do you have multiple monitors, and if so, do you have them all active WHEN YOU LAUNCH Studio One, or are you launching it, then remembering to activate your other monitor(s)?
I have 4 monitors. 2 on my desk, 1 above my 88 key keyboard that I use for placing sheet music / lead sheets on and 1 in my vocal booth. If I forget to turn one on after I launch Stutio One, and then turn it on, the playhead goes out of sync (and alignment as well). All I have to do to fix it is minimize studio one and then maximize it again and it fixes it.
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