Are you using ASIO audio, or Windows audio?

It sounds to me like S1 is setting the sample rate to 44.1K when you start it up and the Windows app is changing it to 48K. When you go back to S1, it's now wrong.

If you use ASIO, it's entirely separate from the Windows audio. Now, I'm not familiar with your interface, but on the ones that I use that's how it works.

If you are using ASIO for S1, then I'm stumped.

j0001s wroteAre you using ASIO audio, or Windows audio

I’m now away from my PC so apologies if this sounds dumb but where/how do I check this. Thanks

I owned a scarlett 6i6 (1st gen) before which had the same behaviour. The device will not switch sample rates as triggered by the DAW. You could check the Focusrite site for beta drivers. A good workaround is to set the device (I think you use MixControl for that) to the desired samplerate and after that start Studio One. That helped for me. I sold my 1st gen scarlett because I got tired or these driver issues. Bought in 2014 and had lots and lots of driver issues. Sound and hardware was very good but bad bad drivers. As up to today they never released an newer official driver for it.

Oh yeah.... I remember now.... Please set you Audio in the Windows configuration panel from 48 (DVD quality) to 44.1 kHz (cd quality) for playback and recording. That might help as well. Even though it's a Windows setting it will determine the sample rate of your device.Samplerate switching was Always a mess with my device.....

Thanks, I'll check the drivers.
Just to confirm it is using ASIO audio

I downloaded and reinstalled the necessary from Focusrite website. This seems to have given me another option to select that wasn’t showing before - ASIO USB2 with Mixcontrol. I think there was just ASIO before. Anyway selecting this may have solved the issue hopefully. I’ll check again later. Thanks

You need to set your ASIO drivers in Studio One to the ASIO for your Focusrite device. I think sample rate is in fact hardware related but of course managed by the drivers. If your windows drivers set it to 48kHz (which was the default setting in my case) your ASIO drivers used in studio one must still set it to 44.1 succesfully. That's where it went wrong in my experience. So if you set your windows audio settings all to 44.1 it is already set to what you want in Studio One and ASIO does not have to do it. But..... of course you will get a problem again if you want to create a 48kHz project in studio one. Also good chance that newer beta drivers will fix your issue.... That you have to try....

Thanks for your advice.
Can you tell me where..or how I go about changing the Windows Drivers settings?
Cheers

It's in the control panel. That will be a lot of click this and click that to explain. Watch the instructions in this link. Lef me know if you cannot manage and i will try to make an instruction for you.

https://youtu.be/ko1A1qcqWWQ